Arg - 27 , Arg - 127 and Arg - 155 in the f - trefoil protein barley x - amylase / subtilisin inhibitor are interface residues in the complex with barley ax - amylase 2
نویسنده
چکیده
Arginine residues in barley a-amylase/subtilisin inhibitor (BASI) involved in binding to barley a-amylase 2 (AMY2) were differentially labelled using AMY2 as protectant and phenylglyoxal (PGO) and [14C]PGO as modifying agents. Chymotryptic fragments of labelled BASI were purified by reverse-phase HPLC, and we concluded that the radiolabelled Arg-27, Arg-155 and most likely Arg-127, identified by amino acid, sequence and 14C analyses, are protected by AMY2. While Arg-106 and Arg-107 showed intermediate reactivity and apparently were only partly accessible, Arg-15, Arg-41 and Arg-61 reacted with PGO and were thus exposed in the BASI-AMY2 complex. Patterns of arginine modification by [14C]PGO in free or in AMY2complexed BASI were consistent with the results of differential
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